Frequency of highly oncogenic HPV genotypes associations in Arad County and their impact on DALY by cervical cancer

Ioana Rucsanda Toma1

Victor Toma2

Dana Gabriela Negru3

Emilian Damian Popovici1

Ana-Liana Tataru4


Victor Babes University of Medicine and Pharmacy Timisoara

County Emergency Clinical Hospital „Pius Brînzeu” Timișoara

Epidemiology Medical Office Dr. Negru Dana, Arad

“Vasile Goldis” Western University of Arad

Objectives. Intercepting the frequency of highly oncogenic HPV genotypes associations in healthy women versus disability-adjusted life year (DALY) by cervical cancer in the same population at the same time period to quantify the disease burden as is described by metrics from mortality and morbidity, meaning  overall disease burden, expressed  specifically as the number of years lost due to ill-health, disability or early death. Hypothese tested: cervical cancer is more frequently in under 35-year-old women compared to other genital cancers and also highly oncogenic HPV genotypes associations are more present in the same age category.

Methods. Molecular diagnostic tests for HPV–DNA of highly oncogenic genotypes were obtained by Polymerase chain reaction method from cervical swap samples, total 250, between 2016-2019 in Arad County, with Cobas 4800 (Roche Diagnostics) automate platform. Cytology-based genital cancers profile and age related relative risk (ARR) for genital cancers were analyzed for theirs quantitative variables, using IBM SPSS Statistic 20 and MedCalc software packages, descriptive and analytical methods for non-randomized, observational, longitudinal retrospective study, being used. For DALY calculation The Global Burden of Disease GBD 2013 methodology was applied.

Results. Of 250 samples 56% were positive for one or more HPV types. Infection in women aged 18-75 years with 1 of 14 high-risk genotypes of human papillomavirus (HR-HPV) was found in more than 52% of samples (P = 0,0001) as listed: types 16, 18, 31, 33, 35, 39, 51, 52, 56, 58, 59, 66, 68, and 82. Low-risk HPV types identified were types 6, 11, 40, 42, 54, 61,62, 81 and 84, of total of 20% of tested women. Odds ratio for high-risk genotypes of human papillomavirus (HR-HPV) in women aged 25-29 years was 6.1 compared to other age categories. Cervical dysplasia was present mainly between the ages of 25 and 35, cervical cancer being diagnosed at 55. DALY per 1000 for cervical cancers was 34.

Conclusion. Different  HPV types are present alone or in association in 56% of tested women aged 18-75 years in Arad County, and 52% of samples were positive for HR-HPV, with no differences between rural or urban residence of the patients. HPV vaccination for women through age 26 years would have been welcomed. Cervical cancer screening has to be done, using the Pap test and HPV test, which are both extremely useful for early detection of risks for and precancerous lesions. The data show that only 11% of cervicitis are identified and treated, and only 24% of treated cases are followed by another required Pap test. For every 1,000 women in Arad, one loses 31 years of life through premature death.

34 IUSTI Congress - European Congres on Sexually transmitted Infections and HIV/AIDS
Bucharest, September 3-5,